| Annotated protein: | Voltage-dependent calcium channel gamma-8 subunit (Neuronal voltage-gated calcium channel gamma-8 subunit) (Transmembrane AMPAR regulatory protein gamma-8) (TARP gamma-8). Gene symbol: CACNG8. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8VHW2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNG8 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Yamasaki M, et al. "TARP gamma-2 and gamma-8 Differentially Control AMPAR Density Across Schaffer Collateral/Commissural Synapses in the Hippocampal CA1 Area" J Neurosci. 2016 Apr 13;36(15):4296-312 PMID:27076426 |
| Figure(s): | Fig 5. G, H, L |
| Annotation description: | TARP γ-8 is a multipass transmembrane protein which is concentrated in the postsynaptic density plasma membrane at four types of Schaffer collateral/commissural (SSC) synapses in the adult mouse hippocampus: perforated and non-perforated synapses on pyramidal cells; axodendritic synapses on parvalbumin-positive (PV) and PV-negative interneurons (Figure 5C, D, J). Among the TARPs examined in this paper (γ-2, γ-3, γ-8), TARP γ-8 was the only TARP found at nonperforated synapses. Antibodies used in this paper were validated by immunofluorescence labeling (Figure 7) and electron microscopy (Table 2-3) using KO mice for the corresponding TARP. 6/12/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Figure 5- PSD prep shows TARPg8 localization in PSD fraction @ PMID:21172611 Figure 6A- PSD prep @ PMID:12771129 |
| SynGO annotation ID: | 2047 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |