| Annotated protein: | Voltage-dependent calcium channel gamma-3 subunit (Neuronal voltage-gated calcium channel gamma-3 subunit) (Transmembrane AMPAR regulatory protein gamma-3) (TARP gamma-3). Gene symbol: CACNG3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JJV5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNG3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Yamasaki M, et al. "TARP gamma-2 and gamma-8 Differentially Control AMPAR Density Across Schaffer Collateral/Commissural Synapses in the Hippocampal CA1 Area" J Neurosci. 2016 Apr 13;36(15):4296-312 PMID:27076426 |
| Figure(s): | Figure 5-6 |
| Annotation description: | TARP γ-3 is one of four classical or type-I TARPs, of which only three are expressed in the hippocampus (γ-2, γ-3, γ-8). TARP γ-3 is multipass transmembrane protein which is concentrated in the in the plasma membrane inside the postsynaptic density (PSD) of axodendritic synapses on parvalbumin-positive (PV) interneurons in the adult mouse hippocampus (Figure 5E, F, K). Examination of other types of Schaffer collateral/commissural (SSC) synapses shows that small amounts of TARP γ-3 protein are found in the PSD of perforated and non-perforated synapses on pyramidal cells and axodendritic synapses on PV-negative interneurons. Antibodies used in this paper were validated by immunofluorescence labeling (Figure 7) and electron microscopy (Table 2-3) using KO mice for the corresponding TARP. Examination of the cellular expression of TARP γ-3 in mouse hippocampal neurons shows TARP γ-3, mRNA is significantly higher in PV-positive interneurons than pyramidal cells (similar pattern to TARP γ-2, Figure 6C-D, H, K, N, Q). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| SynGO annotation ID: | 2046 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |