| Annotated protein: | Voltage-dependent calcium channel gamma-2 subunit (Neuronal voltage-gated calcium channel gamma-2 subunit) (Stargazin) (Transmembrane AMPAR regulatory protein gamma-2) (TARP gamma-2). Gene symbol: CACNG2. Taxonomy: Mus musculus (Mouse). Uniprot ID: O88602 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNG2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Yamasaki M, et al. "TARP gamma-2 and gamma-8 Differentially Control AMPAR Density Across Schaffer Collateral/Commissural Synapses in the Hippocampal CA1 Area" J Neurosci. 2016 Apr 13;36(15):4296-312 PMID:27076426 |
| Figure(s): | Figure 5 |
| Annotation description: | TARP γ-2 is one of four classical or type-I TARPs, of which only three are expressed in the hippocampus (γ-2, γ-3, γ-8). TARP γ-2 is multi-pass transmembrane protein which is concentrated in in the postsynaptic density (PSD) of Schaffer collateral/commissural (SSC) synapses in the adult mouse hippocampus (Figure 5C, D, J). TARP γ-2 is significantly enriched in the PSDs of axodendritic synapses of PV-positive interneurons (compared to PV-negative neurons). TARP γ-2 is also found in the PSDs in pyramidal cells, where TARP γ-2 is preferentially localized to perforated synapses over to non-perforated synapses. The distinct synaptic expression pattern of TARP γ-2 is similar to that of AMPA receptors at SSC synapses (Figure 1, 3-4). Antibodies used in this paper were validated by immunofluorescence labeling (Figure 7) and electron microscopy (Table 2-3) using KO mice for the corresponding TARP. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | This paper defines the family of TARPs (γ-2, γ-3, γ-4, γ-8, cloned cDNAs from rat brain). Western blotting of protein extracts shows the tissue distribution for each TARP (Figure 2A). TARP γ-2 is enriched in the cerebellum, but also found in the cerebral cortex and hippocampus (Figure B-C). Biochemical fractionation shows TARP γ-2, γ-3, γ-4 and γ-8 are all highly enriched in the postsynaptic density fraction (Figure 6A). @ PMID:12771129 |
| SynGO annotation ID: | 2045 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |