| Annotated protein: | Glycine receptor subunit beta (Glycine receptor 58 kDa subunit). Gene symbol: GLRB. Taxonomy: Mus musculus (Mouse). Uniprot ID: P48168 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GLRB |
| Ontology domain: | Biological Process |
| SynGO term: | transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential (GO:1904315) |
| Synapse type(s): | brainstem, glycinergic |
| Annotated paper: | Graham BA, et al. "Distinct physiological mechanisms underlie altered glycinergic synaptic transmission in the murine mutants spastic, spasmodic, and oscillator" J Neurosci. 2006 May 3;26(18):4880-90 PMID:16672662 |
| Figure(s): | Fig.2 |
| Annotation description: | Background: - This paper studies glycinergic responses in motorneurons in the hypoglossal nucleus. - The spastic, spasmodic, and oscillator mice have mutations in resp. beta, alpha1 and alpha1 GlycR genes. - The spastic mouse have a non-coding mutation that expresses lower levels of the beta subunit. Fig.2: "Properties of glycinergic mIPSCs in control (spa/+) and spa/spa mice" Results: "the spastic mutation causes a dramatic reduction in mIPSC amplitude and frequency with no change in mIPSC kinetics" Discussion: "Based on strychnine binding, the spastic mutation reduces GlyR numbers without altering affinity (supplemental Table 1, available at www.jneurosci.org as supplemental material) (White and Heller, 1982; Becker et al., 1986)" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Background: - This paper studies glycinergic responses in motorneurons in the hypoglossal nucleus. - functional ionotropic glycine receptors can consist of only alpha-type subunits (characterized by a high chloride conductance) or of mixed alpha/beta-type subunits (characterized by a low chloride conductance) (see PMID:8404844). Fig.1: With gene expression analysis it is observed that fetal (α2) GlyR subunit mRNA decreased, whereas expression of adult (α1) GlyR subunit mRNA increased postnatally. The ß subunit is expressed at all moments of postnatal development. Fig.2: A postnatal switch in glycinergic inhibitory postsynaptic currents (IPSCs) in brain stem motor neurons is observed. Single channel recordings show that glycine triggered channels with a low (30pS) conductance level that did not change postnatally (data not shown). The conclusion is that during postnatal maturation, ionotropic glycine receptor in the motorneurons change from a α2/beta to a α1/beta subunit composition. @ PMID:9819267 |
| SynGO annotation ID: | 2031 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |