| Annotated protein: | Acetylcholine receptor subunit epsilon. Gene symbol: CHRNE. Taxonomy: Mus musculus (Mouse). Uniprot ID: P20782 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CHRNE |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Synapse type(s): | Neuro-muscular junction |
| Annotated paper: | Porter CW, et al. "The ultrastructural localization and quantitation of cholinergic receptors at the mouse motor endplate" J Membr Biol. 1973;14(4):383-402 PMID:4781450 |
| Figure(s): | Fig.1+2 |
| Annotation description: | Fig.1 " Autoradiograph of a portion of an endplate labeled with 3H-alpha-bungarotoxin. Typically, the grains are distributed over the postjunctional folds" In muscle, alpha-bungarotoxin is a marker of the muscle type nAChR that consists of the α1β1εδ subunits in adult tissue and of the α1β1γδ subunits during fetal development (see attached review in the Additional literature section). 3H-alpha-bungarotoxin is regarded as a marker of all muscle type nAChR subunits. This paper studies adult mice and can be used to annotate all adult nAChR subunits. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | One of the many reviews that discusses the fixed stoichoimetry of the mature muscle-type nAChR (α1β1εδ subunits) and the fetal muscle-type nAChR (α1β1γδ subunits). @ PMID:19546202 |
| SynGO annotation ID: | 2015 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |