| Annotated protein: | Neuronal acetylcholine receptor subunit alpha-4. Gene symbol: CHRNA4. Taxonomy: Mus musculus (Mouse). Uniprot ID: O70174 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CHRNA4 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | striatum, dopaminergic |
| Annotated paper: | Tarren JR, et al. "Acute Ethanol Administration Upregulates Synaptic alpha4-Subunit of Neuronal Nicotinic Acetylcholine Receptors within the Nucleus Accumbens and Amygdala" Front Mol Neurosci. 2017 Oct 24;10:338 PMID:29114204 |
| Figure(s): | Fig.7+8 |
| Annotation description: | Fig.7+8: Authors look at the effect of EtOH exposure on expression and presynaptic localization of the nACHR alpha4 subunit in dopaminergic nerve terminals in the nucleus accumbens. Localization is assessed with a alpha4-GFP knock-in mouse model. Authors quantified presynaptic localization by measuring colocalization of GFP-alpha4 with the presynaptic marker synaptophysin withing HT-positive terminals. 27/11/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 1985 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |