| Annotated protein: | ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (Cool-associated and tyrosine-phosphorylated protein 1) (CAT-1) (CAT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1) (GRK-interactor 1). Gene symbol: GIT1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z272 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GIT1 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645) |
| Synapse type(s): | cerebral cortex, GABAergic |
| Annotated paper: | Smith KR, et al. "GIT1 and betaPIX are essential for GABA(A) receptor synaptic stability and inhibitory neurotransmission" Cell Rep. 2014 Oct 9;9(1):298-310 PMID:25284783 |
| Figure(s): | fig 2 a-c; fig. S4 a-e; fig 6 a-e |
| Annotation description: | Fig 2a-c GIT1 is important for maintaining GABAa receptors at surface. Literal "GIT1 knockdown neurons exhibited a significant decrease in surface GABAAR and VGAT cluster area compared to control (Figures 2A-2C), suggesting a possible role for GIT1 fig S4 a-e GIT1 is essential for inhibitory synaptic transmission Literal "Analysis of spontaneous IPSC (sIPSC) traces from these cells revealed that GIT1 or bPIX knockdown, or inhibition of PAK all caused a considerable decrease in sIPSC amplitude compared to control neurons fig 6 a-e GIT1 is essential for inhibitory synaptic transmission Literal "To further explore the impact of inhibiting the GIT1 signaling pathway, we measured the impact on miniature IPSCs (mIPSCs). We recorded mIPSCs from neurons transfected with control or GIT1 RNAi (Figures 6A-6E). Analysis of traces from these neurons showed that knockdown of GIT1 caused a significant decrease in both mIPSC amplitude and frequency, which could be rescued with RNAi resistant hGIT1, again showing the specificity of the RNAi knockdown." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1949 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |