| Annotated protein: | Nitric oxide synthase 1 (EC 1.14.13.39) (Constitutive NOS) (NC-NOS) (NOS type I) (Neuronal NOS) (N-NOS) (nNOS) (Nitric oxide synthase, brain) (bNOS) (Peptidyl-cysteine S-nitrosylase NOS1). Gene symbol: NOS1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P29476 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NOS1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Burette A, et al. "Synaptic localization of nitric oxide synthase and soluble guanylyl cyclase in the hippocampus" J Neurosci. 2002 Oct 15;22(20):8961-70 PMID:12388603 |
| Figure(s): | fig 3, 4, 6 |
| Annotation description: | NOS-I antibody: Zymed (South San Francisco, CA) (Table 1). Is characterized in this paper (no shown). 21/11/2017 Pim Fig.3: Authors note: "Although the limited resolution of light microscopy prevents definite conclusions, these data are consistent with the hypothesis that sGC concentrates in axon terminals, whereas NOS-I concentrates in postsynaptic densities." Fig.6: "Quantitative EM of randomly selected asymmetric synapses in the stratum radiatum confirmed that gold particles coding for NOS-I were predominantly postsynaptic, concentrating just cytoplasmic to the postsynaptic membrane, whereas particles coding for sGCβ were predominantly presynaptic, concentrating ~15-25 nm cytoplasmic to the presynaptic membrane (Fig. 6 C)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 1948 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |