| Annotated protein: | Gamma-aminobutyric acid receptor subunit beta-1 (GABA(A) receptor subunit beta-1). Gene symbol: GABRB1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P50571 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABRB1 |
| Ontology domain: | Biological Process |
| SynGO term: | G protein-coupled neurotransmitter receptor activity involved in regulation of presynaptic membrane potential (GO:0150047) |
| Synapse type(s): | hippocampus, GABAergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Schuler V, et al. "Epilepsy, hyperalgesia, impaired memory, and loss of pre- and postsynaptic GABA(B) responses in mice lacking GABA(B(1" Neuron. 2001 Jul 19;31(1):47-58 PMID:11498050 |
| Figure(s): | Fig 3 |
| Annotation description: | GABAB1-deficient (GABAB1-/-) mice were generated. Using classical electrophysiological techniques together with application of baclofen, a GABA B agonist, authors showed lost of Pre- and Postsynaptic GABAB Responses in GABA B1 -/- mice. This demonstrates that GABAB1 is an essential component of pre- and postsynaptic GABAB receptors. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Electrophysiology (generic) |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| Additional literature: | Using molecular modeling and site-directed mutagenesis, the authors have identified two residues in lobe I, Ser246 and Asp471, and one residue in lobe II of the GABA-B1 subunit that are critical for agonist binding and activation of the heteromeric receptor. @ PMID:10986293 |
| SynGO annotation ID: | 1941 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |