| Annotated protein: | Tissue-type plasminogen activator (t-PA) (t-plasminogen activator) (tPA) (EC 3.4.21.68) [Cleaved into: Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B]. Gene symbol: PLAT. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P19637 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PLAT |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | brain |
| Annotated paper: | Shin CY, et al. "Rapid, activity-induced increase in tissue plasminogen activator is mediated by metabotropic glutamate receptor-dependent mRNA translation" J Neurosci. 2004 Oct 20;24(42):9425-33 PMID:15496678 |
| Figure(s): | Fig. 1C, 4, 5B |
| Annotation description: | tPA localizes to postsynapes opposite to synapsin (Fig. 1C) in primary hippocampal neurons and is co-enriched with PSD-95 in synaptoneurosome preparations (Fig. 5B). 17/11/2017 Pim - Fig.4: Expression of tPA mRNA in dendrite Figure shows that the tPA protein is expressed by and therefore localized to the denditic compartment. The synaptic localization show in Fig.1 is therefore (at least) postsynaptic. 25/11/2017 eg Evidence for activity-dependent release is provided by the study by Lochner et al. (2005) PMID 16555239. Accordingly the site of action is supposedly the synaptic cleft or the perisynaptic extracellular matrix. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot Biochemical fractionation (generic) |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | Supplemental table S1 contains 492 proteins of the PSD fraction identified either by 2D gel electrophoresis followed by MS/MS or by LC-MS/MS. tPA was among these identified proteins. @ PMID:14720225 This paper shows recombinant tPA in dendritic spines of mature hippocampal neurons in culture (Fig. 2, 3). Upon K+-induced depolarization recombinant tPA is released (Fig. 6) @ PMID:16555239 |
| SynGO annotation ID: | 1905 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |