| Annotated protein: | Phospholipid phosphatase-related protein type 4 (Brain-specific phosphatidic acid phosphatase-like protein 1) (Inactive 2-lysophosphatidate phosphatase PLPPR4) (Lipid phosphate phosphatase-related protein type 4) (Plasticity-related gene 1 protein) (PRG-1). Gene symbol: PLPPR4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q7TME0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PLPPR4 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Trimbuch T, et al. "Synaptic PRG-1 modulates excitatory transmission via lipid phosphate-mediated signaling" Cell. 2009 Sep 18;138(6):1222-35 PMID:19766573 |
| Figure(s): | Fig. 1D |
| Annotation description: | Figure 1: PRG-1 (gene PLPPR4) was mainly expressed on dendrites and colocalized with ProSAP2 (SHANK3) and Homer, but not Gephyrin (GPHN). Subcellular fractionation of mouse cerebral cortex revealed PRG-1 within the PSD. PRG-1 was detected in this fraction but not in the presynaptic vesicle fraction (SC+CSV). "Finally, localization at the ultrastructural level revealed DAB-reaction product, indicating PRG-1 immunolabeling exclusively at postsynaptic structures of asymmetric synapses of CA1 pyramidal cells and silver-enhanced immunogold signal of PRG-1 bordering the synaptic cleft (Figure 1D)." Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | PGR-1 (gene PLPPR4) characterization @ PMID:12730698 |
| SynGO annotation ID: | 1904 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |