| Annotated protein: | GTP-binding protein Rheb (Ras homolog enriched in brain). Gene symbol: RHEB. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62639 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RHEB |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse organization (GO:0099175) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Yasuda S, et al. "Activation of Rheb, but not of mTORC1, impairs spine synapse morphogenesis in tuberous sclerosis complex" Sci Rep. 2014 Jun 3;4:5155 PMID:24889507 |
| Figure(s): | Figure 3 |
| Annotation description: | Figure 3: Rheb regulates spine synapse formation. (a) Dendrites of EGFP- and mock- or FLAG-RhebQ64V-expressing hippocampal neurons prepared from WT and Tsc2+/- rats at DIV 19. Neurons were co-immunostained with anti-GFP and vGlut1 antibodies. Scale bar, 10μm. (b, c) Cumulative probability distributions of protrusion width (b) and length (c) in (a). RhebQ64V-expressing WT neurons showed narrower (D = 0.2690, P < 0.001) and longer (D = 0.2391, P < 0.001) dendritic protrusions than mock transfectants (Kolmogorov-Smirnov test). (d) Quantification of the spine synapse densities shown in (a). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| SynGO annotation ID: | 1896 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |