| Annotated protein: | Neuronal cell adhesion molecule (Nr-CAM) (Neuronal surface protein Bravo) (mBravo) (NgCAM-related cell adhesion molecule) (Ng-CAM-related). Gene symbol: NRCAM. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q810U4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NRCAM |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Demyanenko GP, et al. "Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling" J Neurosci. 2014 Aug 20;34(34):11274-87 PMID:25143608 |
| Figure(s): | Fig. 5D and E. |
| Annotation description: | Fig. 5D and E, the double immunostaining showed colocalization of NrCAM (green) and Npn-2 (red) at numerous puncta (arrows) in confocal images of V1, layer 4 (P21) and dendritic spine puncta of pyramidal cells in V1, layer 4 (P21), labeled with EGFP by in utero electroporation and EGFP-positive, were labeled for both NrCAM and Npn2. Literal: "Double immunofluorescence staining showed colocalization of NrCAM and Npn-2 at numerous puncta in confocal images of mouse brain (P21) in sections of V1, layer 4, consistent with association studies in COS-7 cells (Fig. 5D). It was reported that Npn-2 colocalizes with PSD-95. In Figure 1C, NrCAM staining of puncta in V1, layer 4 (P21) overlapped with the postsynaptic marker PSD-95, while pyramidal cells labeled with EGFP by in utero electroporation showed punctate labeling at the tip of spines for both NrCAM and Npn2 (Fig. 5E). 9/11/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1888 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |