| Annotated protein: | Disintegrin and metalloproteinase domain-containing protein 22 (ADAM 22). Gene symbol: ADAM22. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9R1V6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADAM22 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | brain, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | Fukata Y, et al. "Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission and epilepsy" Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3799-804 PMID:20133599 |
| Figure(s): | Fig. 3b,c, Fig. 4b, Fig. 5d and Fig. S3a |
| Annotation description: | Fig. 3b, using pull-down experiment ADAM23 form a protein complex with LGl1, PSD-95 and and Kv1.2. Literal: "Major constituents of LGI1 complex contained ADAM23 as well as ADAM22 and PSD-95." Fig. 4b, ADAM22 coimmunoprecipitate with PSD-95 and Kv1.2. Literal: "Loss of LGI1 reduced the amount of both ADAM22 and ADAM23 in Triton X-100-insoluble crude synaptic fractions by ~80% and inversely increased in Triton X- 100-soluble fractions (Fig. 5A). This is consistent with the data that the interaction of ADAM22 with postsynaptic PSD-95 is reduced in the LGI1-/- mouse" Fig. 5d, ADAM22 protein localizes in the outer molecular layers of the dentate gyrus Literal: "Our immunohistochemical analysis showed that ADAM23 protein localizes in the outer molecular layers of the dentate gyrus (Fig. 5D), where perforant path fibers from the entorhinal cortex terminate. These results suggest the presynaptic localization of ADAM23 at least in the molecular layer in the dentate gyrus. Given that the LGI1 protein complex contains both presynaptic and postsynaptic proteins (Fig. 3D), it is conceivable that LGI1 mediates the transsynaptic interaction between postsynaptic ADAM22 and presynaptic ADAM23." Fig. S3a ADAM22 is enriched in Triton X-100-soluble presynaptic and insoluble PSD fractions. Literal: "ADAM22, and ADAM23 were enriched both in Triton X-100-soluble presynaptic and insoluble postsynaptic density (PSD) fractions" 7/6/2017 Pim These data show that: (i) ADAM22 is found in dame molecular complex as PSD-95 (ii) That the interaction of ADAM22 and PSD-95 is direct (iii) ADAM22 is enriched in PSD fractions. Note: ADAM22 has a TM domain. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1840 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |