| Annotated protein: | Voltage-dependent calcium channel gamma-4 subunit (Neuronal voltage-gated calcium channel gamma-4 subunit) (Transmembrane AMPAR regulatory protein gamma-4) (TARP gamma-4). Gene symbol: CACNG4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q8VHW9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNG4 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Tomita S, et al. "Functional studies and distribution define a family of transmembrane AMPA receptor regulatory proteins" J Cell Biol. 2003 May 26;161(4):805-16 PMID:12771129 |
| Figure(s): | Figure 6A |
| Annotation description: | TARP γ-4 is an integral membrane protein found in the postsynaptic density membrane. Fig. 6A: "TARPs colocalize with AMPARs at excitatory synapses." Subcellular fractionation and western blot analysis showed TARP γ-4 is enriched in synaptosomes and is resistant to extraction from synaptic membranes with Triton X-100 (PSDI-II fractions), indicating a strong association with the PSD. 'integral component of postsynaptic density membrane (GO:0099061)' is based on (1) the transmembrane topology of the TARP γ-4 and (2) the known interaction with AMPARs (plasma membrane proteins) at the PSD (3) TARP γ-4 regulation the decay of synaptic AMPAR currents in cerebellar granule cells (see BP annotation).. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| SynGO annotation ID: | 1837 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |