Annotated protein:Interleukin-1 receptor accessory protein (IL-1 receptor accessory protein) (IL-1RAcP) (EC 3.2.2.6) (Interleukin-33 receptot beta chain). Gene symbol: IL1RAP. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61730-3
antibody wiki:
SynGO gene info:SynGO data @ IL1RAP
Ontology domain:Biological Process
SynGO term:regulation of presynapse assembly (GO:1905606)
Synapse type(s):cerebral cortex, glutamatergic
Annotated paper:Yoshida T, et al. "Interleukin-1 receptor accessory protein organizes neuronal synaptogenesis as a cell adhesion molecule" J Neurosci. 2012 Feb 22;32(8):2588-600 PMID:22357843
Figure(s):Fig. 3, 4, 8
Annotation description:Fig. 3d,e,f ; siRNA-mediated knockdown of IL-1RAcP isoforms in cortical neurons reduced the staining signals for presynaptic Bassoon and decreased the number of dendritic spines.
Literal:
"The siRNA against Il1rap was transfected into cultured cortical neurons at DIV 10. After 4d of incubation, we observed a significant reduction of staining signals
for presynaptic Bassoon along the dendrites of the transfected cortical neurons and a decrease of number of dendritic protrusions (Fig. 3D-F)"

Fig. 4a,b; transfected FLAG-IL-1RAcP in cortical neurons increased punctate immunostaining signals for Bassoon
Literal:
"Transfection of cultured cortical neurons at DIV 12 with the expression vector for FLAG-IL-1RAcP increased punctate immunostaining signals for Bassoon but exerted little effect on dendritic protrusions (Fig. 4)

Fig.8 IL-1RAcPb and IL-1RAcP (not shown) requires PTPδ for presynaptic differentiation
Evidence tracking, Biological System:Cultured neurons
Non-neuronal tissue
Evidence tracking, Protein Targeting:RNAi / shRNA
Over-expression
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Chiara Verpelli (ORCID:0000-0003-2949-9725)
Carlo Sala (ORCID:0000-0003-0662-9523)
Lab:CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy
SynGO annotation ID:1828
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology