| Annotated protein: | Excitatory amino acid transporter 2 (Glutamate/aspartate transporter II) (Sodium-dependent glutamate/aspartate transporter 2) (Solute carrier family 1 member 2). Gene symbol: SLC1A2. Taxonomy: Homo sapiens (Human). Uniprot ID: P43004 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC1A2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Melone M, et al. "Cellular and Synaptic Localization of EAAT2a in Human Cerebral Cortex" Front Neuroanat. 2011 Jan 14;4:151 PMID:21258616 |
| Figure(s): | Fig. 6, fig. 7a.b |
| Annotation description: | Fig 6 synaptic localization of EAAT2 in human cortex Literal "confocal microscopy of EAAT2a (TRITC)/VGLUT1 (FITC) double labeled fields from layers II/III of areas 7, 8, 10, and 19 showed that red and green puncta were morphologically different and separated; however, in all merged images many puncta were at least partially yellowindicating a spatial overlap of the two signals.Electron microscopy confirmed that EAAT2+ profiles were both in intimate association with asymmetric synapses and far from the synapses (Figure 6B). To better define the organization of synapses expressing EAAT2a, we performed a pre-embedding electron microscopy analysis of asymmetric synapses (n = 130; layers II/III; areas 8 and 10). These studies showed that 67.9 ± 4.0% of EAAT2a expressed at asymmetric synapses was in astrocytic processes, 17.9 ± 3.3% in axon terminals, while 13.4 ± 2.9% was both in astrocytic processes and in axons (Figures 6C-F). Overall, these data indicated that about half of cortical EAAT2a is in the vicinity of asymmetric synapses and that about 70% of the latter is in astrocytic processes." Fig 7synaptic localization of EAAT2 in human cortex Literal "In samples from layers II/III of areas 8, 10, and 46 processed for immunogold post-embedding electron microscopy, gold particles were observed in both distal astrocytic processes (Figure 7A) and axon terminals (Figure 7B)." 6/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| Additional literature: | Fig 5 and 7 confirmed EAAT2 synaptic localization @ PMID:18805467 |
| SynGO annotation ID: | 1819 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |