| Annotated protein: | Traf2 and NCK-interacting protein kinase (EC 2.7.11.1). Gene symbol: TNIK. Taxonomy: Mus musculus (Mouse). Uniprot ID: P83510 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TNIK |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0098696) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Coba MP, et al. "TNiK is required for postsynaptic and nuclear signaling pathways and cognitive function" J Neurosci. 2012 Oct 3;32(40):13987-99 PMID:23035106 |
| Figure(s): | Fig 2, 3 |
| Annotation description: | Fig. 2J, GluA1 is reduced in the PSD lysates of TNiK-/- mice. Literal: "In PSD extracts from hippocampal slices...of TNiK-/- mice, a decrease in AMPAR GluA1 levels (35%, p <0.05; Fig. 2J) was observed." Fig 3, Alteration in the glutamategic hippocampal CA1 region synaptic physiology in TNIK KO mice. Literal: "Since these data show biochemical interactions between TNiK and glutamate receptors, we examined excitatory and inhibitory synaptic transmission and synaptic plasticity in the hippocampus of TNiK-/- mice. NMDAR-and AMPAR mediated synaptic currents, long-term plasticity (LTP and LTD), and sIPSCs and mIPSCs were normal in the hippocampal CA1 region of TNiK-/-] mice (Fig. 3A-F). We noted, however, that evoked fEPSPs were slightly but significantly enhanced at stronger stimulation intensities compared with wt littermates (Fig. 3B; ...). We also observed a significant increase in PPF ... and a significant decrease in the frequency of AMPAR-mediated mEPSCs in TNiK-/- cells... indicating the probability of neurotransmitter release from presynaptic terminals of excitatory synapses may be reduced in TNiK-/- mice (Fig. 3G). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp IP + WB/MSMS |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1813 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |