| Annotated protein: | Nitric oxide synthase 1 (EC 1.14.13.39) (Constitutive NOS) (NC-NOS) (NOS type I) (Neuronal NOS) (N-NOS) (nNOS) (Nitric oxide synthase, brain) (bNOS) (Peptidyl-cysteine S-nitrosylase NOS1). Gene symbol: NOS1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P29476 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NOS1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic specialization, intracellular component (GO:0099091) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Burette A, et al. "Synaptic localization of nitric oxide synthase and soluble guanylyl cyclase in the hippocampus" J Neurosci. 2002 Oct 15;22(20):8961-70 PMID:12388603 |
| Figure(s): | Fig. 6. |
| Annotation description: | Fig. 6, NOS1 is localized at the postsynaptic density of the stratum radiatum of CA1synapses. Literal: "Immunogold particles coding for NOS-I concentrated over the postsynaptic density of axospinous synapses of asymmetric type (Fig. 6A). In contrast, sGCbeta labeling was concentrated over the presynaptic terminals of asymmetric axospinous synapses (Fig. 6B). Quantitative EM of randomly selected asymmetric synapses in the stratum radiatum confirmed that gold particles coding for NOS-I were predominantly postsynaptic, concentrating just cytoplasmic to the postsynaptic membrane, whereas particles coding for sGCbeta were predominantly presynaptic, concentrating 15-25 nm cytoplasmic to the presynaptic membrane (Fig. 6C). The distributions of NOS-I and sGC suggest that the two enzymes might be present on the two sides of the same synapses. To test this hypothesis, we examined sections double labeled using two sizes of gold particles. To control for possible crossreactivity (because the two primary antibodies were raised in the same species), we also studied serial single-labeled sections. Despite the vagaries of these methods, we were able to demonstrate NOS-I-positive PSDs postsynaptic to sGCbeta-positive terminals, using both techniques (Fig. 6D-F)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| Additional literature: | Fig. 2 also shows the ultrastructural localization of NOS1 to postsynaptic densities @ PMID:11160391 |
| SynGO annotation ID: | 1802 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |