| Annotated protein: | Unconventional myosin-VI (Protein twist) (Unconventional myosin-6). Gene symbol: MYO6. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q64331 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MYO6 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic actin cytoskeleton (GO:0098871) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Osterweil E, et al. "A role for myosin VI in postsynaptic structure and glutamate receptor endocytosis" J Cell Biol. 2005 Jan 17;168(2):329-38 PMID:15657400 |
| Figure(s): | Fig. 2, 5, 6 and 7 |
| Annotation description: | Literal (Fig.2): "This indicates that Myo6 interacts with other proteins at the PSD, in addition to F-actin. In contrast, ATP treatment of Triton X-100 (TX)-permeabilized synaptosomes resulted in the release of the majority of Myo6 from the pellet fraction, suggesting that most synaptic Myo6 associates with F-actin alone (Fig. 2 C)" The site of this interaction is postsynaptic because MyoVI 1) binds AMPARs (Fig.6) and 2) mediates endocytosis of AMPAR (postsynaptic NT-receptor) in dendrites (Fig.7/8). Moreover, Myosins are known actin-binding proteins. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Harold MacGillavry (ORCID:0000-0002-6153-3586) Casper Hoogenraad (ORCID:0000-0002-2666-0758) |
| Lab: | Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands |
| SynGO annotation ID: | 1799 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |