| Annotated protein: | Profilin-2 (Profilin II). Gene symbol: PFN2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JJV2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PFN2 |
| Ontology domain: | Biological Process |
| SynGO term: | modification of postsynaptic actin cytoskeleton (GO:0098885) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Michaelsen-Preusse K, et al. "Neuronal profilins in health and disease: Relevance for spine plasticity and Fragile X syndrome" Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):3365-70 PMID:26951674 |
| Figure(s): | Fig.2, 3, 4 |
| Annotation description: | 26/10/2017 Pim - This paper compares the function of PFN1 and PFN2 at synapses. - PFN2a but not PFN1 influences spine motility, synapse function, and spine plasticity - Both PFN2a (reduces actin polymerization) and PFN1 (promotes actin polymerization) regulate actin dynamics in spines - Authors propose that there is a developmental difference between PFN1 and PFN2 since PFN1 is predominanty expressed at P0 and then is downregulated. - PFN1 but not PFN2 is able to rescue the spine morphology phenotype in FXS mouse model (characterized by immature spines). In conclusion: PFN1 seems to affect spine morphology in the developing CNS, whereas PFN2a affects spine morphology and synaptic transmission in the mature CNS. Both do this via modulation of the actin cytoskeleton (Fig.3) - 'modification' term is selected because the reorganization takes place after a cLTP stimulus. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Fluorescence recovery after photobleaching (FRAP) Whole-cell patch clamp |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 1791 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |