| Annotated protein: | Rho-associated protein kinase 2 (EC 2.7.11.1) (Rho-associated, coiled-coil-containing protein kinase 2) (Rho-associated, coiled-coil-containing protein kinase II) (ROCK-II) (p164 ROCK-2). Gene symbol: ROCK2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P70336 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ROCK2 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic actin cytoskeleton organization (GO:0098974) |
| Synapse type(s): | hippocampus, glutamatergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Zhou Z, et al. "A critical role of Rho-kinase ROCK2 in the regulation of spine and synaptic function" Neuropharmacology. 2009 Jan;56(1):81-9 PMID:18718479 |
| Figure(s): | fig 2a, fig 3; fig 5b,c |
| Annotation description: | Fig 2a ROCK2 is involved in hippocampal synaptic function Literal "The sizes of fEPSPs were significantly reduced in ROCK2 knockout mice compared to the wild-type littermates." Fig 3 ROCK2 deletion affects LTP Literal "As shown in Fig. 3A and B, LTP induced by high frequency stimulation (four trains of 100 Hz lasting 1 s each with 20 s intertrain intervals) was significantly reduced in ROCK2 knockout mice compared to the wild-type littermates" Fig 5b,c Synapse deficits in ROCK2 knockout mice Literal "We counted the total number of asymmetric synapses and found that the density was significantly lower in ROCK2"Interestingly, in addition to a reduction in synapse density, the averaged postsynaptic area or spine area was significantly increased in ROCK2 knockout mice" Fig 7c spine deficits in ROCK2 knockout mice Literal "the spine length was significantly increased in ROCK2 knockout mice. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Field recordings |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1788 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |