| Annotated protein: | Neuron-specific calcium-binding protein hippocalcin (P23K). Gene symbol: HPCA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P84076 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HPCA |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Jo J, et al. "Muscarinic receptors induce LTD of NMDAR EPSCs via a mechanism involving hippocalcin, AP2 and PSD-95" Nat Neurosci. 2010 Oct;13(10):1216-24 PMID:20852624 |
| Figure(s): | fig. 3d,h; fig 4c,d; Fig.5 |
| Annotation description: | Fig. 3d,h Hippocalcin is required for the LTDn that can be induced by the activation of muscarinic receptors. Literal "The role of hippocalcin can be explored using a well-characterized dominant-negative protein (GST-Hip2-72), which is an N-terminal truncation that lacks the Ca2+ binding sites15. Treatment with the dominant-negative protein selectively blocked mAChR-LTDn" Fig 4c,d Hip-RNAi blocks CCh-LTDN Literal "Hip-RNAi caused a small reduction of EPSCN, but there were no significant differences in EPSCA between Hip-RNAi-transfected cells and neighboring nontransfected cells"Consistent with the results of our GST-Hip2-72 experiments, transfection of Hip-RNAi blocked CCh-LTDN, whereas CCh-LTDN was routinely induced in simultaneously recorded, neighboring nontransfected cells" 10/11/2017 Pim Fig.5: mAChR-LTDN involves the internalization of NMDARs This figure shows that mAChR-LTDN, regulated by HPCA, involves the dynamin-dependent endocytosis of NMDARs. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1785 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |