| Annotated protein: | ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (Cool-associated and tyrosine-phosphorylated protein 1) (CAT-1) (CAT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1) (GRK-interactor 1). Gene symbol: GIT1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z272 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GIT1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, GABAergic hippocampus, glutamatergic brain, GABAergic |
| Annotated paper: | Smith KR, et al. "GIT1 and betaPIX are essential for GABA(A) receptor synaptic stability and inhibitory neurotransmission" Cell Rep. 2014 Oct 9;9(1):298-310 PMID:25284783 |
| Figure(s): | fig 1a, supplementary fig 1a; fig1b |
| Annotation description: | Fig 1a; Fig S1a GIT1 is localized to GABAercic synaptic sites Neurons labeled with antibodies to GIT1, VGAT (Vesicular GABA transporter) to label inhibitory presynaptic terminals, and the g2 GABAAR subunit to label synaptic GABAAR clusters showed GIT1 was distributed along dendrites and exhibited colocalization with synaptic GABAAR clusters (Figure 1A; Figure S1A) Fig 1b GIT1 is localized to glutamatergic synaptic sites Literal "we confirm by showing that the excitatory synapse marker, homer, demonstrates 60% colocalization with GIT1 puncta (Figure 1B)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1781 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |