Annotated protein:Catenin alpha-2 (Alpha N-catenin). Gene symbol: CTNNA2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61301
antibody wiki:
SynGO gene info:SynGO data @ CTNNA2
Ontology domain:Cellular Component
SynGO term:extrinsic component of presynaptic membrane (GO:0098888)
Synapse type(s):cerebral cortex
cerebellum
Mossy fiber synapse (DG->CA3)
Parallel fibre (cerebellum -> Purkinje-cell)
Annotated paper:Uchida N, et al. "The catenin/cadherin adhesion system is localized in synaptic junctions bordering transmitter release zones" J Cell Biol. 1996 Nov;135(3):767-79 PMID:8909549
Figure(s):Figure 3A, Figure 4C, 4E, 4H, Figure 6, Fig.8
Annotation description:NCAT2 (Hirano et al., 1992), a rat mAb against alphaN-catenin, was used for
production of other mAbs to this protein. alphaN-catenin selective monoclonal antibody is used in immunoperoxidase and immunogold labelling of cerebellum and cortex. The authors highlighted recruitment to symmetrical pre- and post synaptic adherence apositions that tend to border release site. They also show early developmental recruitment of this symmetrical adhesive structures prior to synaptic vesicle clustering.

2/11/2017 Pim
- Based on the interaction with N-cadherin shown in Fig.8 the 'extrinsic component...' variant of membrane association was used.
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
IP + WB/MSMS
Annotator(s):Noa Lipstein (ORCID:0000-0002-0755-5899)
Cordelia Imig (ORCID:0000-0001-7351-8706)
Vincent O'connor (ORCID:0000-0003-3185-5709)
Nils Brose (ORCID:0000-0003-0938-8534)
Lab:Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany
SynGO annotation ID:1776
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology