| Annotated protein: | Neuropilin-1 (A5 protein) (CD antigen CD304). Gene symbol: NRP1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P97333 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NRP1 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynapse organization (GO:0099173) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Morita A, et al. "Regulation of dendritic branching and spine maturation by semaphorin3A-Fyn signaling" J Neurosci. 2006 Mar 15;26(11):2971-80 PMID:16540575 |
| Figure(s): | Fig. 3. |
| Annotation description: | Fig. 3, soluble NRP1 (sNRP-1) including the extracellular domain but lacking the cytoplasmic domains is able to block Sema3A ability to induce PSD-95 clusters formation. Literal: "Sema3A concentration dependently increased the density of the PSD-95 immunoreactive clusters per dendrite (Fig. 3A,B). Sema3A also increased the cluster size of PSD-95 (Fig. 3C). To confirm that the clustering of the postsynaptic marker proteins was associated with spine maturation in culture, we compared images of rhodamine-phalloidin staining and PSD-95 clusters. There was an extensive colocalization of PSD-95 clusters with spine-like protrusions after Sema3A treatment (Fig. 3Ad). To confirm a specific effect of Sema3A, we used the construct of sNRP-1. When sNRP-1 was added at the same time as Sema3A in the culture, the sNRP-1 blocked the promoting effect of Sema3A on PSD-95 cluster formation (Fig. 3C)." 6/11/2017 Pim - Authors note in the introduction: 'NRPs are known binders of semaphorins. NRP-1 is necessary for Sema3A-mediated repulsive guidance events (Raper, 2000; Pasterkamp and Kolodkin, 2003; Fujisawa, 2004)" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1760 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |