| Annotated protein: | Diacylglycerol kinase theta (DAG kinase theta) (EC 2.7.1.107) (Diglyceride kinase theta) (DGK-theta). Gene symbol: DGKQ. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6P5E8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DGKQ |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle endocytosis (GO:1900242) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Goldschmidt HL, et al. "DGKtheta Catalytic Activity Is Required for Efficient Recycling of Presynaptic Vesicles at Excitatory Synapses" Cell Rep. 2016 Jan 12;14(2):200-7 PMID:26748701 |
| Figure(s): | Fig.2-4 |
| Annotation description: | DGKθ KD and KO negatively affects SV endocytosis (measured with synaptophysin-pHluorin) in cultured rat neurons (Fig.2) and in mouse neurons (Fig.4). The DGK activity in brain of DGKθ KO mice is reduced (Fig.3) and this activity is essential to rescue the SV recycling phenotype (Fig.4). Note: DGKθ is a lipid metabolazing enzyme that may change the properties of the plasma membrane.Since not addressed specifically in this paper, I refrain from linking this molecular function of DGKtheta to SV recycling. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Optical physiology Enzyme assay |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| SynGO annotation ID: | 1754 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |