| Annotated protein: | Leucine-rich glioma-inactivated protein 1. Gene symbol: LGI1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JIA1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LGI1 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Fukata Y, et al. "Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission and epilepsy" Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3799-804 PMID:20133599 |
| Figure(s): | Fig. 6 |
| Annotation description: | Fig. 6, LGI1 is essential for AMPAR-mediated synaptic currents in the hippocampus. Literal: "we next examined whether loss of LGI1 affects AMPAR-mediated synaptic transmission in the hippocampus. Two methods were used to measure synaptic AMPAR function. First, we compared the AMPA/NMDA ratio in wild-type (LGI1+/+) and LGI1-/- mice. A clear reduction in this ratio was observed (Fig. 6A). This decrease could result from either a selective increase in NMDA synaptic currents or a selective decrease in AMPA synaptic currents. To distinguish between these alternatives, we recorded miniature excitatory postsynaptic currents (mEPSCs) in the presence of tetrodotoxin. Indeed, a significant decrease in the average amplitude of mEPSCs, but not frequency, was found (Fig. 6 C and D). Finally, we examined paired pulse ratio to determine whether the probability of transmitter release is changed in the LGI1-/- mice. No change in the paired pulse ratio was detected (Fig. 6B). These results indicate that the loss of LGI1 reduces AMPAR-mediated synaptic currents in the hippocampus." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| Additional literature: | Similar observation: Fig. 4 ADAM22 binds LGl1 and regulates the number of synaptic AMPARs @ PMID:16990550 |
| SynGO annotation ID: | 1748 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |