| Annotated protein: | Dystroglycan 1 (Dystroglycan) (Dystrophin-associated glycoprotein 1) [Cleaved into: Alpha-dystroglycan (Alpha-DG); Beta-dystroglycan (Beta-DG)]. Gene symbol: DAG1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q62165 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DAG1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0098696) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Fruh S, et al. "Neuronal Dystroglycan Is Necessary for Formation and Maintenance of Functional CCK-Positive Basket Cell Terminals on Pyramidal Cells" J Neurosci. 2016 Oct 5;36(40):10296-10313 PMID:27707967 |
| Figure(s): | fig. 3 |
| Annotation description: | Fig. 3 d,f Loss of neuronal DG results in minor alterations in GABAergic PSD protein clustering Literal "However, quantification of cluster density and size showed a significant decrease of GABAAR α1 subunit size in cKO accompanied by an increase of GABAAR α2 subunit density (Fig. 3D,F)." Fig 4 Neuronal DG ablation leads to selective loss of markers of CCK-positive basket cell terminals Literal "We found that markers for presynaptic terminals from CCK-positive interneurons were virtually absent in CA1 pyramidal layer of DG cKO mice (Fig. 4A-D,G, I,K)."Synaptotagmin 2 and PV immunolabeling was still present in typical punctate distribution in the pyramidal layer of cKO mice (Fig. 4A-D,H, J ), demonstrating the specific requirement of DG for formation of presynaptic terminals from CCK-positive interneurons. Fig 8 h,i,j Formation and maintenance of CCK-positive basket cell terminals require neuronal DG Literal "Examination of VGluT3-positive terminals at 28 dpi in Cre-expressing regions of CA1 pyramidal layer revealed a moderate but significant reduction of VGluT3 puncta density and size in homozygously floxed mice compared with contralateral side compared with the ipsilateral side of heterozygously floxed mice (Fig. 8H). VGluT3- positive terminals in heterozygous mice were not affected. Compromised VGluT3 immunolabeling was also found at later time points in homozygous mice, and the effect became more prominent with increased time after injection (Fig. 8I, J ). Together, these results provide strong evidence for a role of DG bothin synapse formation and in retrograde trans-synaptic signaling for maintenance of CCK-positive terminals." 9/11/2017 Pim - DAG1 protein is a postsynaptic protein in GABAergic synapses onto hippocampal pyramidal neurons. - Postsynaptic DAG1 is needed for localization of GABARs to the PSD (Fig.3) --> term 'regulation of neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0098696)' - Postsynaptic DAG1 is needed for formation and maintenance of functional axon terminals from CCK-positive basket cells onto hippocampal pyramidal neurons. (Fig.4, 5, 7,8 ,9, 10) --> term 'retrograde trans-synaptic signaling by trans-synaptic protein complex (GO:0098942)' regulates 'presynapse organization' Note: The presynaptic partner protein remains unidentified, but is shown not to be neurexin: "Therefore, DG function for CCK-positive terminals is likely neurexin-independent, which suggests that a novel presynaptic receptor might be involved in this trans-synaptic connection." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1745 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |