| Annotated protein: | Ephrin type-B receptor 1 (EC 2.7.10.1). Gene symbol: EPHB1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8CBF3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPHB1 |
| Ontology domain: | Biological Process |
| SynGO term: | modulation of chemical synaptic transmission (GO:0050804) |
| Synapse type(s): | spinal cord, glutamatergic |
| Annotated paper: | Liu WT, et al. "An in vivo mouse model of long-term potentiation at synapses between primary afferent C-fibers and spinal dorsal horn neurons: essential role of EphB1 receptor" Mol Pain. 2009 Jun 12;5:29 PMID:19523204 |
| Figure(s): | Fig. 5D, Fig. 6 and Fig. 7 |
| Annotation description: | Fig. 5D, LTP of synapses between C-fibers and dorsal horn neurons in mice in vivo is impaired in EPHB1 KO mice. Literal: "our results showed that targeted mutation of the EphB1 receptor completely abolished the LTP in all mice tested (n = 9) (Fig. 5D). These genetic data strongly suggest that this EphB1 receptor is the specific Eph family receptor critical to and required for development of LTP." Fig. 6E-H, high frequency stimulation induced increases of p-CaMKII, p-ERK and p-CREB in dorsal horn neurons is abolished of EPHB1 KO mice. Literal: "Further, targeted mutation of the EphB1 receptor completely blocked HFS induced increases of p-CaMKII, p-ERK (Fig. 6E, F) and p-CREB (Fig. 6G, H)." and Fig. 7, high frequency stimulation induced increases c-Fos expression is reduced in in dorsal horn neurons is abolished of EPHB1 KO mice. Literal: "Similarly, HFS-induced c-Fos expression was significantly reduced by EphB2-Fc (2 μg, from 30 min prior to 3 h after HFS) in WT mice, or prevented by targeted mutation of the EphB1 receptor in the EphB1-/- mice. Representative photomicrographs and the corresponding counts of Fos-like immunoreactive neurons in DH are shown in Fig. 7. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) Field recordings Western blot |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1738 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |