| Annotated protein: | P2X purinoceptor 2 (P2X2) (ATP receptor) (Purinergic receptor). Gene symbol: P2RX2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P49653 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ P2RX2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Rodrigues RJ, et al. "Dual presynaptic control by ATP of glutamate release via facilitatory P2X1, P2X2/3, and P2X3 and inhibitory P2Y1, P2Y2, and/or P2Y4 receptors in the rat hippocampus" J Neurosci. 2005 Jul 6;25(27):6286-95 PMID:16000618 |
| Figure(s): | Fig. 5C |
| Annotation description: | The paper defines pharmacological finger print to implicate P2Y receptors in modulation of transmitter release. Selected figures independently show subcellular fractionation/western blotting and immunocytochemical staining of isolated synaptosomes. Using commercial antibodies characterized for selective signal upon recombinant expression of cDNA the authors evidence both pre (Figure 5C) and post (6B) synaptic localization. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | Similar biochemical and immunocytochemical staining to evidence localization at GABAergic synapses. @ PMID:18468677 |
| SynGO annotation ID: | 1737 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |