| Annotated protein: | CaM kinase-like vesicle-associated protein. Gene symbol: CAMKV. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q3UHL1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CAMKV |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic structure (GO:0099159) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Liang Z, et al. "The pseudokinase CaMKv is required for the activity-dependent maintenance of dendritic spines" Nat Commun. 2016 Oct 31;7:13282 PMID:27796283 |
| Figure(s): | fig 2; fig 5 |
| Annotation description: | Fig 2 CaMKv regulates dendritic spine density through Ca2+/ calmodulin. literal "CaMKv knockdown by either shRNA significantly reduced dendritic spine density (Fig. 2b,c; Supplementary Fig. 2b,c). Co-transfection of RNA interference (RNAi)-resistant CaMKv constructs with the shRNA in cultured neurons rescued the reduced spine density, suggesting that the observed spine defects were due to CaMKv depletion (Fig. 2b,c)."Consistent with spine density reduction, CaMKv knockdown reduced mEPSC frequency" Fig 5 CaMKv knockdown in vivo reduces spine density and impairs basal synaptic neurotransmission and L-LTP Literal "Consistent with the findings in dissociated hippocampal neurons, CaMKv shRNA virus injection into the mouse hippocampus significantly decreased spine density in CA1 neurons compared with the control virus injection (Fig. 5e,f). Extracellular field recording of CA1 neurons also revealed attenuated synaptic transmission indicated by the ratio of the field excitatory postsynaptic potential (fEPSP)amplitude to fibre volley (Fig. 5g). Therefore, CaMKv function in spine maintenance contributes to normal synaptic transmission in mouse hippocampus. we investigated whether CaMKv contributes to L-LTP. L-LTP was significantly attenuated after CaMKv knockdown, while NMDA receptor-dependent long-term depression was unchanged (Fig. 5h,i; Supplementary Fig. 5c).These findings suggest that CaMKv is required for both synaptic transmission and protein synthesis-dependent LTP in the hippocampus. 9/11/2017 Pim - The term 'maintenance' used in this paper means that CaMKv is needed to keep the spine shape intact after LTP stimuli. I therefore selected term 'regulation of modification of postsynaptic structure (GO:0099159)' where modification represents the response to stimulus. - The authors show effects on basic ST and LTP: "CaMKv is required for synaptic transmission and late-phase LTP". This can be translated into a term "modification of synaptic transmission" |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp Field recordings |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1724 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |