| Annotated protein: | Adhesion G protein-coupled receptor B1 (Brain-specific angiogenesis inhibitor 1) [Cleaved into: Vasculostatin-120 (Vstat120); Vasculostatin-40 (Vstat-40)]. Gene symbol: ADGRB1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: C0HL12 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADGRB1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Duman JG, et al. "The adhesion-GPCR BAI1 regulates synaptogenesis by controlling the recruitment of the Par3/Tiam1 polarity complex to synaptic sites" J Neurosci. 2013 Apr 17;33(16):6964-78 PMID:23595754 |
| Figure(s): | fig. 1 b,d, e, f, g |
| Annotation description: | Fig 1 BAI1 is expressed in neurons and localizes to excitatory synapses. 1b Literal "We posited that BAI1 might be synaptogenic because of its presence in dendritic spines, apparent even in a low-magnification image of a mature hippocampal neuron" 1d Literal "Comparing the gradient of BAI1 staining from spine heads to immediately adjacent segments of dendritic shaft revealed that BAI1 was enriched in spine heads to an extent similar to PSD95, whereas acetylated tubulin was enriched in dendritic shafts and EGFP filled both regions equally. 1e Literal "Strikingly, even overexpressed BAI1 targeted strongly to spines 1f Literal "First, we biochemically purified synapses and found that BAI1 was enriched in the PSD fraction, although to a lower extent than PSD95 1g Literal "Second, we stained mature cultured neurons for BAI1 and the synaptic markers PSD95 or GluR1 (Fig. 1G). In both cases, BAI1 colocalized significantly with these markers in spines, indicative of synaptic localization." 9/11/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. - Postsynaptic localization is directly shown in the paper. Term 'integral component of postsynaptic membrane (GO:0099055)' was inferred from TM domains and known rol in cell adhesion. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1721 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |