| Annotated protein: | Neuromodulin (Axonal membrane protein GAP-43) (Growth-associated protein 43) (Protein F1). Gene symbol: GAP43. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P07936 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GAP43 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic specialization assembly (GO:0099150) |
| Synapse type(s): | cerebral cortex, GABAergic |
| Annotated paper: | Wang CY, et al. "Protein kinase C-dependent growth-associated protein 43 phosphorylation regulates gephyrin aggregation at developing GABAergic synapses" Mol Cell Biol. 2015 May;35(10):1712-26 PMID:25755278 |
| Figure(s): | fig. 1A and D ; fig. 3; fig. 5 |
| Annotation description: | Fig. 1 Inhibition of PKC activity induced GAP43 interaction with gephyrin in developing cortical neurons. Literal "We found that GAP43-gephyrin interaction was markedly increased up to 4.8-fold in cultured neurons treated with the PKC inhibitor Ro318220 for 24 h" Fig 3 GAP43-S41 mutants differentially interacted with gephyrin and affected gephyrin aggregation in cortical neurons. Literal "Together, these results suggest that dephosphorylated GAP43 at S41 strongly interacts with gephyrin and triggers gephyrin aggresome formation, leading to the protection of physiological gephyrin clusters. In contrast, an increase of phosphorylated GAP43-S41 may lead to the negative regulation of gephyrin clustering in developing neurons." Fig 5 Gephyrin knockdown and GAP43S41D overexpression reduced surface GABAAR expression. Literal "Thus, these data suggested that the surface expression of GABAARs in developing cortical neurons is gephyrin dependent and can be negatively regulated by the phosphorylated GAP43- S41, which inhibited gephyrin clustering. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp IP + WB/MSMS |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1720 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |