Annotated protein:Disks large-associated protein 4 (DAP-4) (PSD-95/SAP90-binding protein 4) (SAP90/PSD-95-associated protein 4) (SAPAP-4). Gene symbol: DLGAP4. Taxonomy: Mus musculus (Mouse). Uniprot ID: B1AZP2
antibody wiki:
SynGO gene info:SynGO data @ DLGAP4
Ontology domain:Cellular Component
SynGO term:postsynaptic specialization (GO:0099572)
Synapse type(s):spinal cord, glutamatergic
brain, glutamatergic
peripheral nervous system, acetylcholinergic
Neuro-muscular junction
Annotated paper:Welch JM, et al. "Differential mRNA expression and protein localization of the SAP90/PSD-95-associated proteins (SAPAPs) in the nervous system of the mouse" J Comp Neurol. 2004 Apr 19;472(1):24-39 PMID:15024750
Figure(s):Fig. 5; Fig. 8; Fig.9; Fig. 11
Annotation description:Fig.5 SAPAP 4 is enriched in PSD
Literal "We found that SAPAP1, -3, and -4 were all highly enriched in PSD fractions."

Fig. 8 SAPAP 4 is expressed at neuronal cholinergic synapses.
Literal "SAPAP1, -3, and -4 were highly enriched at synapses in the submandibular ganglion, as demonstrated by simultaneously staining the sections for the presynaptic marker protein synaptophysin (Fig. 8B,D,F).

Fig. 11 SAPAP 4 proteins colocalizes with PSD-95.
Literal " As a positive control, the same experiments were performed
using double labeling with the NMDA receptor binding protein, PSD-95. SAPAP1, -3, and -4 all showed extensive colocalization with PSD-95 (Fig. 11), implying,
as expected, that SAPAP1, -3, and -4 were located at glutamatergic synapses in the spinal cord."
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Western blot
Annotator(s):Chiara Verpelli (ORCID:0000-0003-2949-9725)
Carlo Sala (ORCID:0000-0003-0662-9523)
Lab:CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy
SynGO annotation ID:1716
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology