Annotated protein: | Disks large-associated protein 4 (DAP-4) (PSD-95/SAP90-binding protein 4) (SAP90/PSD-95-associated protein 4) (SAPAP-4). Gene symbol: DLGAP4. Taxonomy: Mus musculus (Mouse). Uniprot ID: B1AZP2 |
antibody wiki: | |
SynGO gene info: | SynGO data @ DLGAP4 |
Ontology domain: | Cellular Component |
SynGO term: | postsynaptic specialization (GO:0099572) |
Synapse type(s): | spinal cord, glutamatergic brain, glutamatergic peripheral nervous system, acetylcholinergic Neuro-muscular junction |
Annotated paper: | Welch JM, et al. "Differential mRNA expression and protein localization of the SAP90/PSD-95-associated proteins (SAPAPs) in the nervous system of the mouse" J Comp Neurol. 2004 Apr 19;472(1):24-39 PMID:15024750 |
Figure(s): | Fig. 5; Fig. 8; Fig.9; Fig. 11 |
Annotation description: | Fig.5 SAPAP 4 is enriched in PSD Literal "We found that SAPAP1, -3, and -4 were all highly enriched in PSD fractions." Fig. 8 SAPAP 4 is expressed at neuronal cholinergic synapses. Literal "SAPAP1, -3, and -4 were highly enriched at synapses in the submandibular ganglion, as demonstrated by simultaneously staining the sections for the presynaptic marker protein synaptophysin (Fig. 8B,D,F). Fig. 11 SAPAP 4 proteins colocalizes with PSD-95. Literal " As a positive control, the same experiments were performed using double labeling with the NMDA receptor binding protein, PSD-95. SAPAP1, -3, and -4 all showed extensive colocalization with PSD-95 (Fig. 11), implying, as expected, that SAPAP1, -3, and -4 were located at glutamatergic synapses in the spinal cord." |
Evidence tracking, Biological System: | Intact tissue |
Evidence tracking, Protein Targeting: | Antibody (detection) |
Evidence tracking, Experiment Assay: | Confocal Western blot |
Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
SynGO annotation ID: | 1716 |
Dataset release (version): | 20231201 |
View annotation as GO-CAM model: |