| Annotated protein: | Disks large-associated protein 1 (DAP-1) (Guanylate kinase-associated protein) (rGKAP) (PSD-95/SAP90-binding protein 1) (SAP90/PSD-95-associated protein 1) (SAPAP1). Gene symbol: DLGAP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97836 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DLGAP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Tao-Cheng JH, et al. "Differential distribution of Shank and GKAP at the postsynaptic density" PLoS One. 2015 Mar 16;10(3):e0118750 PMID:25775468 |
| Figure(s): | fig 3, 4 |
| Annotation description: | Fig 3 GKAP is closely associated with the dense material at the core of the PSD complex Literal: "Pre-embedding immunogold electron microscopy was conducted on hippocampal cultures, using antibodies for GKAP and Shank. GKAP antibody was against a C-terminal peptide containing the binding site for Shank, while pan Shank antibody was raised against a peptide that includes the sequence for its PDZ domain, the binding site for GKAP. PSDs of asymmetric synapses showed prominent labeling for both GKAP and Shank (Fig. 3). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 1695 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |