| Annotated protein: | Phosphatidylinositol-binding clathrin assembly protein (Clathrin assembly lymphoid myeloid leukemia protein) (rCALM). Gene symbol: PICALM. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O55012 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PICALM |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of presynaptic endocytic zone membrane (GO:0098894) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Yao PJ, et al. "Synaptic distribution of the endocytic accessory proteins AP180 and CALM" J Comp Neurol. 2005 Jan 3;481(1):58-69 PMID:15558718 |
| Figure(s): | figures 4, 5, 7 |
| Annotation description: | In figures 4, 5, and 7 the authors use immunogold electron microscopy to localize CALM in rat synapses. Figure 4 is synapses from CA1 neurone and figure 5 is synapses from cerebellar granule cells on Purkinje neurons. Figure 7 also uses the granule cell synapses. The immunogold labelling localizes to the pre synapse and figure 7 shows a pool on clathrin-coated vesicles. 6/12/2017 Pim - The localization to the presynaptic endocytic zone is inferred from the following information: 1. PICALM has a well established role in clathrin coat assembly during endocytosis in non-neuronal system. 2. Next to the active zone is a (stucturally poorly defined) hotspot of SV endocytosis names the 'presynaptic endocytic zone'. Fig.4K hows localization of PICALM to a corresponding region bordering the AZ. 3. Localization among clathrin-coated SV is observed (Fig.4C,D,...) These data suggest that (at least in part of its function lifecycle) PICALM is localized to the presynaptic endocytic zone membrane. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 1693 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |