| Annotated protein: | Neurabin I (Neural tissue-specific F-actin binding protein) (Protein phosphatase 1, regulatory subunit 9A). Gene symbol: PPP1R9A. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q7TN74 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPP1R9A |
| Ontology domain: | Biological Process |
| SynGO term: | modulation of chemical synaptic transmission (GO:0050804) |
| Synapse type(s): | striatum |
| Annotated paper: | Allen PB, et al. "Distinct roles for spinophilin and neurabin in dopamine-mediated plasticity" Neuroscience. 2006 Jul 7;140(3):897-911 PMID:16600521 |
| Figure(s): | Fig2-5 |
| Annotation description: | Fig3B shows that Neurabin-1 null mice are impaired in HFS-LTP induction in striatal neurons, but not in LTD induction (Fig3A). The impairment can be restored by a D1 agonist. This suggests modulation of the postsynaptic receptors, probably AMPA through PP-1 (see support ref). This is also supported by data in Fig2 that show that AMPA current rundown is normal, but the D1-mediated AMPA current increase is impaired in Neurabin-1 null neurons. Fig4 and Fig5 show that striatal neurabin KO have in increased mini frequency, with normal mEPSC amplitude. This suggests that also on the presynaptic side, neurotransmission has been altered. This is in accordance of the both pre- and post-synaptic localisation of Neurabin-1. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | This paper shows that a Neurabin-1 mutant that is defective in recruiting protein phosphatase-1 to the synapse (Fig1) affects AMPA receptor surface pool (Fig5) and smaller mini amplitude and frequency (Fig6). @ PMID:17460080 We demonstrated that neurabin KO mice showed a deficit in contextual fear memory but not auditory fear memory. @ PMID:18183288 |
| SynGO annotation ID: | 1682 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |