| Annotated protein: | Large ribosomal subunit protein eL22 (60S ribosomal protein L22) (Heparin-binding protein HBp15). Gene symbol: RPL22. Taxonomy: Mus musculus (Mouse). Uniprot ID: P67984 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RPL22 |
| Ontology domain: | Biological Process |
| SynGO term: | translation at presynapse (GO:0140236) |
| Synapse type(s): | retina, glutamatergic |
| Annotated paper: | Shigeoka T, et al. "Dynamic Axonal Translation in Developing and Mature Visual Circuits" Cell. 2016 Jun 30;166(1):181-92 PMID:27321671 |
| Figure(s): | Fig. 2, 3 |
| Annotation description: | The paper uses the RiboTag-mouse (epitope tagged mouse RPL22) to visualize axonal translation in the visual system. Fig. 1 shows the localization of ribosomes in axon terminals of the superior colliculus. Furthermore the paper characterizes two distinct transcriptomes and transcriptome changes in axon terminals and the soma. 13/10/2017 Pim - Direct role of RPL22 is not show in this paper, but based on well known role of the protein in translation and the author intent. - The direct relevance for synapses is not shown directly, but inferred from analysis of the types of mRNA's that are present in axonal ribosomes. Literal: "Reassuringly, analysis with the cellular component category showed that axon-enriched mRNAs generally encode proteins that are already known to function in axons, growth cones, and ***synapses*** (Figures 3B and andS3B).S3B). In contrast, mRNAs encoding nuclear proteins (e.g., modifier of chromatin structures) are depleted from axonal translatome." (emphasis added) - The 'modulating synaptic transmission' part of selected term 'regulation of translation at presynapse, modulating synaptic transmission (GO:0099577)' is not shown in the paper. We will see if a term 'regulation of translation at presynapse' can be implemented in the SynGO slim and this ticket will be addapted accordingly. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | IP + DNA sequencing |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 1680 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |