| Annotated protein: | Septin-11. Gene symbol: SEPTIN11. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B3GNI6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SEPTIN11 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse organization (GO:0050807) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Li X, et al. "Septin 11 is present in GABAergic synapses and plays a functional role in the cytoarchitecture of neurons and GABAergic synaptic connectivity" J Biol Chem. 2009 Jun 19;284(25):17253-17265 PMID:19380581 |
| Figure(s): | Fig5 and Fig6 |
| Annotation description: | Fig 5 shows that upon Septin 11 knockdown the number of dendritic protrusions is decreased in hippocampal neurons while their length is increased (Fig5B). This suggests a defect in mature spine formation. That this morphological difference also results in less synapses is shown for GABA, Fig5D shows that the number of postsynaptic GABA receptor clusters is decreased upon Septin 11 knock down, and also the overlap between GAD (presysnapse) and gamma2 (postsynapse), indicating that less synapses are formed. This is fully restored by reexpressing Septin 11, and not found in the shRNA with mutations that should not bind to Septin 11 message. These results are further verified by overexpression of a dominant negative Septin 11 that is linked to mCherry. The untagged Septin 11 overexpression has no effect (fig6A-E). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 1631 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |