| Annotated protein: | Septin-7 (CDC10 protein homolog). Gene symbol: SEPTIN7. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9WVC0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SEPTIN7 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynapse organization (GO:0099173) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Tada T, et al. "Role of Septin cytoskeleton in spine morphogenesis and dendrite development in neurons" Curr Biol. 2007 Oct 23;17(20):1752-8 PMID:17935993 |
| Figure(s): | Fig3 and Fig4 |
| Annotation description: | Fig 3 shows that when overexpressed SEPT7 induces an increase in dendritic protrusions (Fig3D), while the length and width of the protrusions remain unaltered. When SEPT7 is knocked down, the length and width of the protrusions increase (Fig4A-C), this can be restored by expressing an shRNA-insensitive clone of SEPT7. These data are also replicated by an other lab, see additional reference. The density of dendritic protrusions in not affected by SEPT7 knock-down (Fig4D). This is different from the observations by Xie et al (PMID 17935997) who observed a reduction in protrusion density upon SEPT7 knockdown or mutant SEPT7 overexpression (PMID 17935997 Fig4), which would be in line with the increase of protrusions upon SEPT7 overexpression observed here in Fig3. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | In this paper also a reduction of spines is observed upon SEPT7 knock-down or overexpression of a SEPT7 mutant in cultured rat hippocampal neurons (Fig4B). Spines here are also longer and thicker (Fig4D, E). @ PMID:17935997 |
| SynGO annotation ID: | 1621 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |