| Annotated protein: | Septin-11. Gene symbol: SEPTIN11. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B3GNI6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SEPTIN11 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | cerebellum, glutamatergic |
| Annotated paper: | Li X, et al. "Septin 11 is present in GABAergic synapses and plays a functional role in the cytoarchitecture of neurons and GABAergic synaptic connectivity" J Biol Chem. 2009 Jun 19;284(25):17253-17265 PMID:19380581 |
| Figure(s): | Fig1, Fig3 and Fig4 |
| Annotation description: | Fig1: Septin 11 migrates with the "type II" PSD fraction in cellular fractionation experiments that is also positive for gephyrin, but negative for PSD-95, suggesting these are GABAergic PSDs. This is verified by confocal microscopy in Fig3, where colocalization of septin 11 with GAD terminals is observed (54±4%), more then with vGlut1 synapses (25±2%; numbers from text p17257). Similar numbers are found in cerebellum (Fig3O-S). This is further verified by 2 methods of immunoEM (pre- and post-embedding; Fig4) in the cerebellum where Septin 11 is found in the dendrite an spine neck of synapses (Fig4A-G and 4J-K), and in the spine and PSD-area of some type II symmetric synapses (Fig4H-I ). Labelling on was absent on the presynaptic side or in axons (text p17258). Note: note that this localisation to glutamatergic synapses is a minority (25%) of the general localisation. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Biochemical fractionation (generic) |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 1616 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |