| Annotated protein: | Synaptotagmin-1 (Synaptotagmin I) (SytI) (p65). Gene symbol: SYT1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P21707 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYT1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of synaptic vesicle membrane (GO:0030285) |
| Synapse type(s): | cerebellum Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Matthew WD, et al. "Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue" J Cell Biol. 1981 Oct;91(1):257-69 PMID:7298720 |
| Figure(s): | Fig. 2, 4, 5 |
| Annotation description: | In this paper, the authors raise two monoclonal antibodies against a protein of 65 000 Da molecular weight (p65 or synaptotagmin-1), which specifically label synaptic vesicle membranes in nerve terminals and are used for immunoisolation of vesicular structures with characteristics of SVs from brain homogenates. The paper represents the first report on synaptotagmin-1 identified in a screen for antibodies against SV proteins. 5/2/2018 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Microscopy (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | The authors report on the structural organization of synaptotagmin-1 and identify N-terminal intravesicular domain with glycosylation signals followed by a TMR and cytosolic C-terminal protion of the protein containg linker regions and two protein kinase C-homologous sequences named C2A and C2B domains. Furthermore, the authors show that the protein co-immunopurifies with SV marker synaptophysin I during immunobead isolation of SVs from rat brain. @ PMID:1985919 Perin et al study the cDNAs encoding human and Drosophila synaptotagmins in order to compare them structurally and functionally. In addition, the authors unambiguously name the p65 protein synaptotagmin 1. Moreover, the structural conservation and similarity between rat, human and Drosophila synaptotagmin 1 suggests conserved function of this SV protein. @ PMID:1840599 Takamori et al perform subcellular fractionation of a rat brain and isolate pure CPG-SVs, which are subjected to advanced characterization by immunoblotting (of all fractions) and mass spectrometry. The proteomic approach unambiguously identifies syt-1 on SVs and the immunodetection of the protein in all fractions adds another line of evidence for presence on SVs. Furthermore, quantification of some of the SV proteins reveals that synaptotagmin-1 counts for about 7% of total SV protein (Table 1,Fig. 1B, Fig. S3). @ PMID:17110340 |
| SynGO annotation ID: | 1609 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |