| Annotated protein: | Alpha-internexin (Alpha-Inx). Gene symbol: INA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P23565 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ INA |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | forebrain, glutamatergic cerebral cortex, glutamatergic |
| Annotated paper: | Suzuki T, et al. "Excitable membranes and synaptic transmission: postsynaptic mechanisms. Localization of alpha-internexin in the postsynaptic density of the rat brain" Brain Res. 1997 Aug 8;765(1):74-80 PMID:9310396 |
| Figure(s): | Fig3, Fig6 |
| Annotation description: | Fig 3 shows that alpha-internexin concentrates in the PSD fraction upon cell fractionation of rat forebrain. This is verified by immuno-EM (DAB method) in Figure 6 where staining is only observed at the post-synaptic side, the presynaptic side remaining relatively clean. No quantification is offered. The authors comment "Immunoreactivity [of alpha-internexin) was clearly localized in the dendrites, spines and in most but not all PSDs (Fig. 6). Presynaptic structures were not stained. The specificity of the staining was confirmed by the lack of stainings with non-immune rabbit immunoglobulins (not shown)." p78 This seems also to hold in mouse brain, see additional reference. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Biochemical fractionation (generic) |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Supplementary figure S2 panel h shows immuno-gold alpha-internexin label in the post-synapse in control mice (so different species then main ref), while the TKO (also lacking alpha-internexin) does not show any label as quantified in panel i. @ PMID:25869803 |
| SynGO annotation ID: | 1607 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |