| Annotated protein: | Gamma-aminobutyric acid type B receptor subunit 1 (GABA-B receptor 1) (GABA-B-R1) (GABA-BR1) (GABABR1) (Gb1). Gene symbol: GABBR1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z0U4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABBR1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | hippocampus, GABAergic hippocampus, glutamatergic |
| Annotated paper: | Kulik A, et al. "Subcellular localization of metabotropic GABA(B) receptor subunits GABA(B1a/b) and GABA(B2) in the rat hippocampus" J Neurosci. 2003 Dec 3;23(35):11026-35 PMID:14657159 |
| Figure(s): | Fig 2, 4, 5-7 |
| Annotation description: | Using preembedding immunohistochemical methods combined with quantitative analysis of GABAB receptor subunit immunoreactivity, this study provides a detailed description of the cellular and subcellular localization of GABAB1a/b and GABAB2 in the rat hippocampus: 1) GABAB1a/b and GABAB2 are colocalized and likely to form heterodimerized functional GABAB receptors in hippocampal neurons. 2) Both subunits were observed on presynaptic and, more abundantly, on postsynaptic elements 3) Presynaptic GABAB receptor subunits are found to be localized to GABAergic and putative glutamatergic axon terminals. The subunits are detected at the extrasynaptic membrane but also at the presynaptic membrane specialization. 4) The majority of postsynaptic subunits were localized to the extrasynaptic membrane of dendritic spines and shafts of principal cells and interneurons. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Microscopy (generic) |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 1588 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |