Annotated protein:	Voltage-dependent L-type calcium channel subunit alpha-1D (Calcium channel, L type, alpha-1 polypeptide isoform 2) (Voltage-gated calcium channel subunit alpha Cav1.3). Gene symbol: CACNA1D. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q99246
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SynGO gene info:	SynGO data @ CACNA1D
Ontology domain:	Cellular Component
SynGO term:	integral component of presynaptic active zone membrane (GO:0099059)
Synapse type(s):	Cochlear hair cell ribbon synapse
Annotated paper:	Brandt A, et al. "Few CaV1.3 channels regulate the exocytosis of a synaptic vesicle at the hair cell ribbon synapse" J Neurosci. 2005 Dec 14;25(50):11577-85 PMID:16354915
Figure(s):	Fig 1
Annotation description:	confocal immunofluorescence using antibodies to detect Cav1.3 and co-stained for the presynaptic (ribbon synapse) protein Ribeye. This staining was completely absent in a Cav1.3 KO mouse. 20/11/2017 Pim - Annotation supported by remark by the authors; "To approximate the number of CaV1.3 channels per active zone, we first estimated the number of ribbon synapses per IHC (Khimich et al., 2005) by counting juxtaposed pairs of RIBEYE-labeled ribbons and postsynaptic glutamate receptor-immunoreactive spots in 3D reconstructions from confocal sections (Fig. 1G)." - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen.
Evidence tracking, Biological System:	Intact tissue
Evidence tracking, Protein Targeting:	Antibody (detection)
Evidence tracking, Experiment Assay:	Confocal
Annotator(s):	Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548)
Lab:	Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA
SynGO annotation ID:	1585
Dataset release (version):	20231201
View annotation as GO-CAM model: