| Annotated protein: | Regulating synaptic membrane exocytosis protein 2 (Rab-3-interacting molecule 2) (RIM 2) (Rab-3-interacting protein 2). Gene symbol: RIMS2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9EQZ7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RIMS2 |
| Ontology domain: | Biological Process |
| SynGO term: | structural constituent of active zone (GO:0098882) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Kaeser PS, et al. "RIM proteins tether Ca2+ channels to presynaptic active zones via a direct PDZ-domain interaction" Cell. 2011 Jan 21;144(2):282-95 PMID:21241895 |
| Figure(s): | Figure 5 |
| Annotation description: | RIM1/RIM2 knockout neurons display a reduction in action potential-induced presynaptic calcium influx (Figure 5). RIM1 acts by regulating the presynaptic localization of voltage-gated calcium channels (Figure 8), mediated by its PDZ domain (Figure 7). Due to their high homology, RIM1 and RIM2 are assumed to be redundant for this process. 23/01/2017 Pim - effect on Ca influx show in Fig.5 - effect on VGCC localization shown in Fig.8 - effect on synaptic transmission shown in Fig.3 10/2/2017 Pim: Note: in Fig.8 the IMP of RIM1/RIM2 KO is rescued with overexpression of RIM1; the IMP is really at the level of both RIM1 and RIM2, the RIM1-rescue is merely a control that demostrated specificty of the observed effect. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | RIM2 knockout neurons display a reduction in calcium-induced vesicle fusion in the Calyx of Held. @ PMID:25343783 RIM2 knockout neurons display a reduction in calcium-induced vesicle fusion in both hippocampal cultures. @ PMID:22753485 |
| SynGO annotation ID: | 157 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |