| Annotated protein: | Protein kinase C zeta type (EC 2.7.11.13) (nPKC-zeta). Gene symbol: PRKCZ. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P09217 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRKCZ |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Hernandez AI, et al. "Cellular and subcellular localization of PKMzeta" Philos Trans R Soc Lond B Biol Sci. 2013 Dec 2;369(1633):20130140 PMID:24298142 |
| Figure(s): | Figure 3 |
| Annotation description: | Using immuno electron microscopy, it is shown that PKM-zeta (a splice variant of the PRKCZ gene) is present at the PSD. No staining was observed in the presynaptic terminal. The authors claim that the staining is no present in KO controls, but do not provide the data for this. 13/3/2017 Pim: - Used antibody does not differentiate between PKCzeta and PKMzeta (isoform of PKCzeta gene). Authors state: "PKMζ mRNA and protein are expressed almost exclusively in brain, whereas the full-length PKCζ mRNA and protein are not expressed in forebrain, except in the lateral olfactory tract [3,5,6]. Therefore, in hippocampus and neocortex, immunostaining with antisera to ζ will specifically determine the localization of PKMζ." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 153 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |