| Annotated protein: | 5-hydroxytryptamine receptor 7 (5-HT-7) (5-HT7) (5-HT-X) (GPRFO) (Serotonin receptor 7). Gene symbol: HTR7. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P32305 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HTR7 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | spinal cord |
| Annotated paper: | Doly S, et al. "Pre- and postsynaptic localization of the 5-HT7 receptor in rat dorsal spinal cord: immunocytochemical evidence" J Comp Neurol. 2005 Sep 26;490(3):256-69 PMID:16082681 |
| Figure(s): | Fig 6, Fig 7 |
| Annotation description: | Under the electron microscope, the immunolabelling of 5-HT7 was localized to cell bodies and dendrites (Fig. 6). - Labeled cell bodies were found in the upper part of lamina III, but also in lamina II and to a lesser extent in lamina I. Staining was localized on different cytoplasmic organelles, such as rough endoplasmic reticulum and vesicles. In addition, the soma contained a number of immunoreactive large granular vesicles (Fig. 6A). - In dendrites, the immunolabelling often covered a group of several centrally located neurotubules (Fig. 6B). When the dendrite was postsynaptic to axon terminals, the staining was often observed at the synaptic differentiation (Fig. 6C). Labeled dendrites have been found postsynaptic to small, unlabeled axon terminals having morphological features of 5-HT terminals. In dendrites plasma membrane labelling is always associated with postsynaptic densities. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| Additional literature: | The antibody used for 5-HT7 labels HeLa cells transfected with the 5-HT7 gene but not untransfected cells. Western blot analysis has shows a single band of 70 kD, compatible with the size of the cloned molecule. @ PMID:9928261 Fig. 1. Immunofluorescence demonstration of 5-HT7 receptor protein in transfected HeLa cells (A), and lack of staining in wild type HeLa cells (B). @ PMID:11173221 |
| SynGO annotation ID: | 1485 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |