| Annotated protein: | 5-hydroxytryptamine receptor 7 (5-HT-7) (5-HT7) (5-HT-X) (GPRFO) (Serotonin receptor 7). Gene symbol: HTR7. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P32305 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HTR7 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | spinal cord |
| Annotated paper: | Doly S, et al. "Pre- and postsynaptic localization of the 5-HT7 receptor in rat dorsal spinal cord: immunocytochemical evidence" J Comp Neurol. 2005 Sep 26;490(3):256-69 PMID:16082681 |
| Figure(s): | Fig 5 |
| Annotation description: | Under the electron microscope, the immunolabelling of 5-HT7 was localized to axons and axon terminals (Figs. 5-8). Axons were primarily thin and unmyelinated, but numerous thin myelinated axons were also observed (Fig. 5A,B). Two main types of axon terminals were observed: large axon terminals, mainly in lamina I, and smaller axon terminals, found preferentially in lamina II. Large axon terminals were long (up to several micrometers) and sinuous (Fig. 5C) or oblong. Axon terminal labelling is rarely associated with the membrane. In small terminals, the staining is often restricted to large granular vesicles containing neuropeptides. It has been suggested that the release of neuropeptides (under sustained primary afferent fiber stimulation) will allow the insertion of the receptor to the membrane. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| Additional literature: | The antibody used for 5-HT7 labels HeLa cells transfected with the 5-HT7 gene but not untransfected cells. Western blot analysis has shows a single band of 70 kD, compatible with the size of the cloned molecule. @ PMID:9928261 Fig. 1. Immunofluorescence demonstration of 5-HT7 receptor protein in transfected HeLa cells (A), and lack of staining in wild type HeLa cells (B). @ PMID:11173221 |
| SynGO annotation ID: | 1484 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |